General strategy

Preliminary statement : The "Environmental Samples" database is not an identification database. All the other databases may be used.

For each of the genes usually used for identification purpose two Bibi Data Bases have been constructed :

a nomenclature stringent containing only sequences from prokariotes having a status in nomenclature.
a lax database containing all the Gen Bank sequences, even if the names have not been precisely set.

Usually identification is performed at first using the stringent database. If identification is of low quality or not possible, you can try the lax database.

Identification strategies using SSU rDNA (16S) databases

Usually identification is performed at first using the stringent database. If identification is of low quality or not possible, you can try the lax database.
If identification is not possible due to an excess of closely related sequences (some month ago over 800 identical sequences for Neisseria meningitidis were in the database) or if no sequences belonging to an external species or genus exists, then try to use the TS database (Type Strains Data Base).

Identification Strategies

General strategy

Identification strategies using SSU rDNA (16S) databases